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KMID : 1007520080170020367
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Food Science and Biotechnology
2008 Volume.17 No. 2 p.367 ~ p.372
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Regulatory Roles of Chrysanthemum zawadskii Roots in Nuclear Factor E2-related Factor 2/Antioxidant Response Element Pathway
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Kang Hye-Sook
Park Min-Ji
Jin Kyong-Suk
Kim Young-Hun
Jun Mi-Ra
Lim Ho-Jin
Jo Wan-Kuen
Kim Jeong-Sang
Jeong Woo-Sik
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Abstract
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Cellular protection against carcinogens could be achieved by the induction of phase 2 detoxifying and antioxidant enzymes such as glutathione S-transferase (GST), NAD(P)H:quinone oxidoreductase 1 (NQO1) and heme oxygenase 1 (HO-1). Nuclear transcription factor E2-related factor 2 (Nrf2) binds to antioxidant response element (ARE) in the promoter region of these genes and the resulting transactivation occurs. In the present study the effect of gujeolcho (Chrysanthemum zawadskii) roots on the Nrf2-ARE pathway were investigated. C. zawadskii root extract was fractionated with a series of organic solvents and their ability to induce Nrf2-ARE pathway was examined. We separated the most potent dichloromethane (DCM) fraction into 12 sub-fractions and found several sub-fractions with strong effects on the Nrf2-ARE pathway. Fraction 4 strongly induced the ARE-reporter gene activity as well as Nrf2 expression. Sitosterol was isolated as a major compound in fraction 4 although its activity was not as potent as its mother fraction. These results indicate that C. zawadskii roots might be used as a potential natural chemopreventive source.
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KEYWORD
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nuclear factor E2-related factor 2, Nrf2, antioxidant response element, chemoprevention, Chrysanthemum zawadskii, sitosterol
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